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Interleukin‐1 stimulates de novo synthesis of mitogen‐activated protein kinase in glomerular mesangial cells
Author(s) -
Huwiler Andrea,
Pfeilschifter Josef
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00754-3
Subject(s) - mitogen activated protein kinase , mitogen activated protein kinase kinase , kinase , microbiology and biotechnology , mesangial cell , protein kinase a , map kinase kinase kinase , ask1 , chemistry , map2k7 , immunoprecipitation , mitogen activated protein kinase 3 , biology , cyclin dependent kinase 2 , biochemistry , in vitro , gene
Interleukin‐1 (IL‐1) stimulates a lime‐ and concentration‐dependent mitogen‐activated protein (MAP) kinase activation in rat mesangial cells. A rapid increase in activity (maximal at 10 min) is followed by a second persistent level of activity which steadily increases over 24 h. The second peak of MAP kinase activity is paralleled by a marked de novo synthesis of p42 MAP kinase as measured by immunoprecipitation of [ 35 S]methionine‐labelled mesangial cells and by a 60% increase in total p42 MAP kinase protein as detected by Western blot analysis. We propose that IL‐1 induced de novo synthesis of p42 MAP kinase is important for the multiplicity of long‐term actions of this cytokine in renal mesangial cells.