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Sulfide dehydrogenase is identical with the SoxB protein of the thiosulfate‐oxidizing enzyme system of Paracoccus denitrificans GB17
Author(s) -
Schneider Angela,
Friedrich Cornelius
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00732-2
Subject(s) - paracoccus denitrificans , thiosulfate , biochemistry , sulfide , chemistry , polyacrylamide gel electrophoresis , enzyme , gel electrophoresis , sulfur , organic chemistry
Thiosulfate induced cells of Paracoccus denitrificans GB17 oxidize thiosulfate and sulfide to sulfate. A mutant carrying a Tn5‐ mob insertion in the soxB gene is unable to oxidize thiosulfate or sulfide suggesting a linkage of both activities. To test this assumption we have separated the components of the thiosulfate‐oxidizing enzyme system of the wild‐type by ion exchange chromatography. The SoxB protein coeluted with a highly active sulfide dehydrogenase. Analysis by polyacrylamide gel electrophoresis revealed one major protein of M r 32k. Thus, the SoxB protein appeared to be identical with sulfide dehydrogenase.