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Nystatin changes the properties of transporters for arginine and sugars An in vitro study
Author(s) -
Opekarová Miroslava,
Tanner Widmar
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00730-6
Subject(s) - nystatin , ergosterol , chemistry , vesicle , biochemistry , efflux , arginine , protonophore , membrane , permease , transporter , vibrio cholerae , biophysics , biology , amino acid , bacteria , antibiotics , genetics , gene
In ergosterol‐containing energized yeast plasma membrane vesicles nystatin (5–10 μg/mg total lipid) caused a massive efflux of pre‐accumulated arginine while the membrane potential (the principal driving force; −110 mV) decreased by only 10–30 mV. Neither the substrate fluxes nor the membrane potential was influenced by nystatin when the permease was reconstituted in ergosterol‐free phospholipid vesicles. The same effect of nystatin was found with the reconstituted sugar transporter front Chlorella kessleri . It is suggested that nystatin binding to ergosterol in the vicinity of the permease releases the transport protein from its coupling to energy and converts it to a facilitator.