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Expression of different mitogen‐regulated protein/proliferin mRNAs in Ehrlich carcinoma cells
Author(s) -
Gil-Torregrosa Beatriz,
Urdiales José L.,
Lozano José,
Mates José M.,
Sanchez-Jimenez Francisca
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00698-9
Subject(s) - orfs , open reading frame , in vivo , microbiology and biotechnology , complementary dna , biology , gene , messenger rna , transcription (linguistics) , gene expression , exon , genetics , peptide sequence , linguistics , philosophy
Results from in vivo and from serum‐free primary cultures of Ehrlich cells suggest that the expression of mitogen‐regulated protein/proliferin (MRP/PLF) mRNAs is not essential for proliferation of this murine tumor. Two sizes for MRP/PRL‐related open reading frames (ORFs) have been detected by reverse transcription/PCR amplification. They are almost identical to that reported for PLF‐1; but 20% of the amplified cDNA included a shorter ORF, which lacks the entire sequence corresponding to that of the exon 3 of the mrp/plf genes. Ehrlich carcinoma may represent a good model to study regulation of expression and physiological roles of MRP/PLFs in vivo.