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The N‐terminal region of the α‐subunit of the TRAP complex has a conserved cluster of negative charges
Author(s) -
Hartmann Enno,
Prehn Siegfried
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00693-8
Subject(s) - endoplasmic reticulum , protein subunit , trap (plumbing) , chromosomal translocation , cluster (spacecraft) , membrane , chemistry , biophysics , biology , topology (electrical circuits) , microbiology and biotechnology , biochemistry , physics , gene , mathematics , combinatorics , meteorology , computer science , programming language
The α‐subunit of the TRAP complex (TRAPα) is a single‐spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAPα in eukaryotes as indicated by its existence in man, fish and plants. Despite the fact that the sequence homology is much lower than for other proteins in the translocation site, the overall topology, the location of the glycosylation sites and, most interestingly, the distribution of charges are conserved. These data indicate that the TRAP complex has a ubiquitous function.