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Transfer RNA binding protein in the nucleus of Saccharomyces cerevisiae
Author(s) -
Wilhelm M.L.,
Reinbolt J.,
Gangloff J.,
Dirheimer G.,
Wilhelm F.X.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00683-0
Subject(s) - saccharomyces cerevisiae , transfer rna , cytoplasm , yeast , rna , dna , nucleus , rna binding protein , biochemistry , microbiology and biotechnology , biology , electrophoretic mobility shift assay , chemistry , gene , gene expression
A yeast nuclear protein that binds to tRNA was identified using a RNA mobility shift assay. Northwestern blotting and N‐terminal sequencing experiments indicate that this tRNA‐binding protein is identical to zuotin which has previously been shown to bind to Z‐DNA [(1992) EMBO J. 11, 3787–37961. Labeled TRNA and poly(dG‐m 5 dC) stabilized in the Z‐DNA form identify the same protein on a Northwestern blot. In a gel retardation assay poly(dG‐m 5 dC) in the Z‐form strongly diminishes the binding of tRNA to zuotin. These studies establish that zuotin is able to bind to both tRNA and Z‐DNA. Zuotin may be transiently associated with tRNA in the nucleus of yeast cells and play a role in its processing or transport to the cytoplasm.