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Metastatic potential of transformed rat 3Y1 cell lines is inversely correlated with lysosomal‐type sialidase activity
Author(s) -
Miyagi Taeko,
Sato Kaoru,
Hata Keiko,
Taniguchi Shun'ichiro
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00682-2
Subject(s) - sialidase , sialic acid , chemistry , glycoprotein , transformation (genetics) , lysosome , enzyme , cell culture , biochemistry , microbiology and biotechnology , biology , neuraminidase , gene , genetics
We have investigated sialidase activities in transformed rat 3Y1 cells of different metastatic potential. Only lysosome‐type sialidase was apparent in the particulate fractions of 3Y1 cells and their transformants. As compared with control 3Y1 cells, src ‐transformed cells exhibited decreased sialidase activity, and v‐ fos transfer to these latter induced even more severe decrease in the sialidase activity with acquisition of high lung metastatic ability. Various lysosomal enzymes other than sialidase were hardly affected by the transformation. Sialic acid transfer to N ‐linked glycoproteins was slightly elevated in the transformants, but not in parallel with their metastatic potential.