Premium
6‐Fluoropyridoxol: A novel probe of cellular pH using 19 F NMR spectroscopy
Author(s) -
Mehta Vimal D.,
Kulkarni Padmakar V.,
Mason Ralph P.,
Constantinescu Anca,
Aravind Sivasubramanian,
Goomer Naresh,
Antich Peter P.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00675-x
Subject(s) - nuclear magnetic resonance spectroscopy , spectroscopy , chemistry , nuclear magnetic resonance , analytical chemistry (journal) , physics , stereochemistry , chromatography , quantum mechanics
6‐Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a p K a ∼8.2 appropriate for physiological investigations. Using 19 F NMR spectroscopy we determined both intra‐ and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion‐electrodes and 31 P NMR spectroscopy.