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Identification of the essential yeast protein MIM17, an integral mitochondrial inner membrane protein involved in protein import
Author(s) -
Maarse Ammy C.,
Blom Jolanda,
Keil Petra,
Pfanner Nikolaus,
Meijer Michiel
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00669-5
Subject(s) - translocase of the inner membrane , mitochondrial carrier , translocase of the outer membrane , atp–adp translocase , inner mitochondrial membrane , inner membrane , vesicle associated membrane protein 8 , mitochondrion , saccharomyces cerevisiae , integral membrane protein , microbiology and biotechnology , biology , biochemistry , mitochondrial membrane transport protein , protein targeting , yeast , membrane protein , bacterial outer membrane , gene , membrane , escherichia coli
We analyzed four Saccharomyces cerevisiae mutants defective in mitochondrial protein import and found that they are complemented by a novel gene encoding a 17 kDa protein. The protein is integrally located in the mitochondrial inner membrane and is termed MIM17. It shows significant homology to MIM23/Mas6p, a previously identified mitochondrial inner membrane protein required for the import of preproteins. Like MIM23, the precursor of MIM17 is synthesized without a presequence. A deletion of MIM17 is lethal. MIM17 thus joins the small group of mitochondrial proteins that are essential for the viability of yeast. We propose that MIM17 is an essential component of the preprotein import machinery of the mitochondrial inner membrane.