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Subtypes of inositol 1,4,5‐trisphosphate receptor in human hematopoietic cell lines: Dynamic aspects of their cell‐type specific expression
Author(s) -
Sugiyama Tomoyasu,
Yamamoto-Hino Miki,
Miyawaki Atsushi,
Furuichi Teiichi,
Mikoshiba Katsuhiko,
Hasegawa Mamoru
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00662-8
Subject(s) - biology , receptor , cell type , inositol , haematopoiesis , microbiology and biotechnology , cell culture , extracellular , complementary dna , northern blot , cell , inositol trisphosphate receptor , expression cloning , cell surface receptor , gene , stem cell , biochemistry , genetics
Inositol 1,4,5‐trisphosphate (IP 3 )‐mediated Ca 2+ signaling plays important roles in cellular responses to extracellular stimuli. We recently succeeded in cloning human counterparts of the three subtypes derived from separate genes. Using the cDNA sequences type‐specific to these subtype receptors, we here analyzed the expression profile of IP 3 R subtypes in stimulated and unstimulated human hematopoietic cell lines representing T cells, B cells, neutrophils, macrophages, erythrocytes and megakaryocytes. Northern and dot blot analysis showed that each IP 3 R subtype is expressed differently in these cells and that the expression profile in each cell is dynamically changed upon stimuli which induce differentiation. Moreover, most of these cells were found to simultaneously express at least two different subtype receptors.