Premium
Recoverin mediates the calcium effect upon rhodopsin phosphorylation and cGMP hydrolysis in bovine retina rod cells
Author(s) -
Gorodovikova Ele.,
Gimelbrant Alexandr A.,
Senin Ivan I.,
Philippov Pavel P.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00661-x
Subject(s) - recoverin , rhodopsin , phosphorylation , chemistry , biophysics , calcium , phosphodiesterase , retina , kinase , biochemistry , microbiology and biotechnology , retinal , biology , enzyme , neuroscience , organic chemistry
Rhodopsin phosphorylation and in consequence cGMP hydrolysis in bovine rod outer segments are Ca 2+ dependent in the presence of ATP. The level of rhodopsin phosphorylation decreases and the lifetime of active phosphodiesterase increases when the free [Ca 2+ ] is raised from <1 nM to about 1 μM; in both cases the half‐maximal effect was observed at 140–170 nM of free Ca 2+ . Antibodies to recoverin reverse both effects at high [Ca 2+ ] but have no influence at low [Ca 2+ ]. We conclude that the Ca 2+ effects observed are mediated by recoverin which inhibits rhodopsin kinase at a high Ca 2+ level.