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The human ubiquitin‐conjugating enzyme UbcH1 is involved in the repair of UV‐damaged, alkylated and cross‐linked DNA
Author(s) -
Kaiser Peter,
Mansourl Hamdy A.,
Greeten Tim,
Auer Bernhard,
Schweiger Manfred,
Schneider Rainer
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00656-3
Subject(s) - dna repair , saccharomyces cerevisiae , ubiquitin conjugating enzyme , complementation , dna , ubiquitin , biology , dna damage , enzyme , biochemistry , function (biology) , xeroderma pigmentosum , ubiquitin ligase , microbiology and biotechnology , yeast , genetics , gene , phenotype
The human ubiquitin‐conjugating enzyme UbcH1 shows 69% identity to the Saccharomyces cerevisiae RAD6/UBC2 which plays a key role in DNA repair. To examine the function of UbcH1 (formerly named E2, M , 17 000), [(1990) EMBO J. 9, 1431–1435]) we tested its ability to functionally substitute for yeast RAD6/UBC2 in the recovery of cells from various DNA damage. Complementation by expression of the human UbcH1 cDNA revealed that the UbcH1 carries out the function of S. cerevisiae RAD6/UBC2 in the repair of UV‐damaged, alkylated and cross‐linked DNA.