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Hydroxyl radical generation in skeletal muscle atrophied by immobilization
Author(s) -
Kondo Hisao,
Nishino Kohsuke,
Itokawa Yoshinori
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00641-5
Subject(s) - radical , chemistry , hydroxyl radical , skeletal muscle , in vivo , hindlimb , medicine , biochemistry , biology , microbiology and biotechnology
Male Wistar rats (15 weeks old), the ankle joints of one hindlimb of which were immobilized in the extended position for 12 days, were injected with salicylate as a trapping agent for hydroxyl radicals before sacrifice. Atrophied and contralateral soleus, typical slow red muscles were collected and their levels of 2,3‐dihydroxybenzoic acid (2,3‐DHB), one of the main reaction products formed by the attack of hydroxyl radical on salicylate, were determined using high‐performance liquid chromatography with an electrochemical detector. There was a significant increase in 2,3‐DHB in the atrophied muscle. This result strongly suggests the enhanced generation in vivo of hydroxyl radicals in atrophied muscle.