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Enzyme‐aided construction of medium‐sized alditols of complete O‐linked saccharides
Author(s) -
Maaheimo Hannu,
Penttilä Leena,
Renkonen Ossi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00638-5
Subject(s) - bacteroides fragilis , enzyme , chemistry , glycan , stereochemistry , biochemistry , in vitro , glycoside hydrolase , glycoprotein , antibiotics
We have constructed by enzyme‐aided in vitro synthesis a hexasaccharide alditol Galβ1—4GlcNAcβ1—6Galβ1—4GlcNAcβ1—6(Galβ1—3)GalNAc‐ol and shown that it resists the action of endo‐β‐galactosidase from Bacteroides fragilis under conditions where a related pentasaccharide alditol, GlcNAcβ1—3Galβ1—4GlcNAcβ1—6(Galβ1—3)GalNAc‐ol, was completely cleaved. Together with earlier results from this laboratory, our present data imply that endo‐β‐galactosidase from B. fragilis , apparently, can be used to distinguish between GlcNAcβ1—6Gal and GlcNAcβ1—3Gal units within linear backbone sequences of all known types of oligo‐( N ‐acetyllactosamino)glycans.