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Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae
Author(s) -
van Berkel M.A.A.,
Caro L.H.P.,
Montijn R.C.,
Klis F.M.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00631-8
Subject(s) - saccharomyces cerevisiae , cell wall , biochemistry , chemistry , cell , agglutinin , fusion protein , recombinant dna , yeast , gene , lectin
Extension of a reporter protein with the carboxyterminal thirty amino acids of the cell wall mannoprotein α‐agglutinin of Saccharomyces cerevisiae resulted in incorporation of the chimeric protein in the cell wall. By Western analysis it was shown that the incorporated protein contained β‐1,6‐glucan similar to endogenous cell wall proteins, whereas excreted reporter protein was not glucosylated. This suggests that β‐1,6‐glucan is involved in anchoring mannoproteins in the cell wall.