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Formation of F 2 ‐isoprostanes during aortic endothelial cell‐mediated oxidation of low density lipoprotein
Author(s) -
Gopaul N.K.,
Nourooz-Zadeh J.,
Mallet A.I.,
Änggård E.E.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00628-8
Subject(s) - isoprostanes , isoprostane , chemistry , arachidonic acid , low density lipoprotein , lipid peroxidation , lipoprotein , high density lipoprotein , endocrinology , medicine , oxidative stress , biochemistry , chromatography , enzyme , cholesterol , biology
We investigated the formation of F 2 ‐isoprostanes produced by non‐enzymatic peroxidation of arachidonic acid during rabbit aortic endothelial cell‐mediated oxidation of low density lipoprotein (LDL). Free and total (sum of free and esterified) levels of F 2 ‐isoprostanes were measured using a solid‐phase extraction procedure and gas chromatography‐mass spectrometry. Free levels of F 2 ‐isoprostanes in native LDL were 0.06 ± 0.03 ng/mg protein ( n = 4), whereas total levels were 0.28 ± 0.09 ng/mg protein ( n = 4). Both free and total levels of the isoprostanes were found to increase during the oxidation. 8‐epi‐PGF 2α was the major isoprostane formed (free and total concentrations after 24 h, 2.50 ± 0.24 and 6.42 ± 1.36 ng/mg protein ( n = 4), respectively). The release of F 2 ‐isoprostanes during aortic endothelial cell‐induced oxidation of LDL could be a contributory factor in the development of atherosclerosis.