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Priming effect of hydroxyapatite on the chemiluminescence response in human polymorphonuclear leukocytes
Author(s) -
Nagase Mitsuo,
Nishiya Hajime,
Noda Masatoshi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00626-1
Subject(s) - chemiluminescence , zymosan , chemistry , stimulation , ionophore , phorbol , granulocyte , calcium , neutrophile , sodium fluoride , biochemistry , pharmacology , endocrinology , immunology , in vitro , phosphorylation , chromatography , protein kinase c , medicine , fluoride , inorganic chemistry , membrane , organic chemistry
In order to determine whether hydroxyapatite modulates the response of polymorphonuclear leukocyte (PMN) to oxidative stimuli, human PMNs were incubated with a non‐activating concentration (1 or 10 μg/ml) of hydroxyapatite prior to stimulation with N ‐formyl‐methionyl‐leucyl‐phenylalanine (FMLP; 0.1 or 1 μM), phorbol 12‐myristate 13‐acetate (PMA; 100 pg/ml), sodium fluoride (50 μM), zymosan (1 μg/ml), or the calcium ionophore A23187 (0.1 μM). Chemiluminescence was measured with an automatic microcomputer‐controlled luminescence analyzer at 37°C. Hydroxyapatite alone did not stimulate chemiluminescence at concentrations below 10 μg/ml. Levels 300–400% higher than ‘stimulus only’ controls without preincubation with hydroxyapatite have been recorded. This synergism between hydroxyapatite and subsequent stimuli reveals a new activity of hydroxyapatite and suggests that particulate material may prepare PMNs for an exaggerated inflammatory response to other phlogistic mediators. This is the first report demonstrating PMNs primed with particulate material.