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In vivo incorporation of cobalt into Propionibacterium shermanii superoxide dismutase
Author(s) -
Meier Beate,
Sehn Anja P.,
Sette Marco,
Paci Maurizio,
Desideri Alessandro,
Rotilio Giuseppe
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00624-5
Subject(s) - propionibacterium , superoxide dismutase , cobalt , chemistry , in vivo , propionibacterium freudenreichii , biochemistry , biology , bacteria , enzyme , microbiology and biotechnology , genetics , organic chemistry , fermentation
Propionibacterium shermanii , an aerotolerant anaerobic bacterium, has already been shown to incorporate, depending on the metal supplementation to the medium, either iron or manganese or copper into the same superoxide dismutase protein. The in vivo incorporation of cobalt in the same superoxide dismutase was obtained in an iron‐, manganese‐ and copper‐depleted medium. The protein was isolated and characterized by NMR which offers the possibility to identify the amino acid residues at the active site exploiting isotropically shifted proton resonance.