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Increased expression of nucleoside diphosphate kinases/nm23 in human diploid fibroblasts transformed by SV40 large T antigen or 60 Co irradiation
Author(s) -
Ohneda Kinuko,
Fukuda Mitsugu,
Shimada Nobuko,
Ishikawa Naoshi,
Ichou Tateo,
Kaji Kazuhiko,
Toyota Takayoshi,
Kimura Narimichi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00623-7
Subject(s) - wi 38 , kinase , cell culture , immortalised cell line , microbiology and biotechnology , fibroblast , sv40 large t antigen , antigen , biology , nucleoside , cell growth , chemistry , biochemistry , ploidy , transfection , immunology , gene , genetics
When the expression levels of nucleoside diphosphate (NDP) kinase/nm23 were examined in four human normal diploid fibroblast cell lines in comparison with their corresponding immortalized cells transformed by SV40 large T antigen or 60 Co irradiation, mRNA levels of the two isoforms (NDP kinase A/nm23‐H1, NDP kinase B/nm23‐H2) were increased in the immortalized cell lines. The increase was found to be associated with increased translation products. Furthermore, the cell extracts prepared from these immortalized cell lines demonstrated slightly higher enzyme activity than those from their normal counterparts. Neither the growth state nor the in vitro aging largely affected their expression in a normal cell line (TIG‐3) examined. The results suggest possible involvement of NDP kinases/nm23 in acquiring an infinite growth property of these cells.