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Molecular cloning of a bovine MSH receptor which is highly expressed in the testis
Author(s) -
Vanetti Mirko,
Schönrock Christiane,
Meyerhof Wolfgang,
Höllt Volker
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00619-9
Subject(s) - microbiology and biotechnology , biology , receptor , transfection , molecular cloning , amino acid , messenger rna , northern blot , southern blot , gene , homology (biology) , genomic dna , western blot , neuron derived orphan receptor 1 , complementary dna , nuclear receptor , biochemistry , transcription factor
A novel G protein‐coupled receptor (BDF3) was isolated from bovine genomic DNA by a combined approach of polymerase chain reaction (PCR) and hybridization techniques. The predicted amino acid sequence is 317 amino acids in length and displays 80% homology to the human α‐MSH receptor MC1. Stably transfected into CHO‐K1 cells, BDF3 mediates an increase of intracellular cAMP‐levels following incubation with NLE‐α‐MSH, a potent α‐MSH analog. The stimulation with ACTH 1–10 is only moderate and γ‐MSH is ineffective. Northern blot analysis of bovine tissues revealed that the BDF3 gene is higly expressed in the testis as a single 2.3 kb mRNA species, suggesting an involvement of the BDF3 receptor in spermatogenesis.