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Dependence of thymocyte apoptosis on protein kinase C and phospholipase A 2
Author(s) -
Shaposhnikova Vera V.,
Dobrovinskaya Oksana R.,
Eidus Lazar Kh.,
Korystov Yuri N.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00616-4
Subject(s) - protein kinase c , ask1 , mitogen activated protein kinase kinase , protein kinase a , phospholipase a2 , activator (genetics) , biology , apoptosis , map kinase kinase kinase , map2k7 , microbiology and biotechnology , cyclin dependent kinase 9 , phospholipase , c raf , phospholipase c , phorbol , kinase , cyclin dependent kinase 2 , biochemistry , signal transduction , enzyme , receptor
The effect of inhibitors and activators of protein kinase C and phospholipase A 2 on radiation‐induced apoptosis of rat and mouse thymocytes has been studied. It is shown that the apoptosis is prevented by the protein kinase C inhibitor 1‐(5‐isoquinolinylsulfonyl)‐2‐methylpiperasine dihydrochloride and is potentiated by protein kinase C activator phorbol 12‐myristate 13‐acetate, calcium ionophore A23187 and concanavalin A. The protein kinase C activators initiate apoptosis in mouse but not in rat thymocytes. The inhibitor of phospholipase A 2 prevents apoptosis induced by all the factors. The results obtained indicate that both protein kinase C and phospholipase A 2 are involved in the thymocyte apoptosis.