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Cloning a novel human brain inward rectifier potassium channel and its functional expression in Xenopus oocytes
Author(s) -
Tang Weimin,
Yang Xian-Cheng
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00612-1
Subject(s) - xenopus , inward rectifier potassium ion channel , potassium channel , complementary dna , amino acid , microbiology and biotechnology , messenger rna , cloning (programming) , cdna library , transmembrane domain , extracellular , peptide sequence , biology , chemistry , biophysics , ion channel , gene , biochemistry , receptor , computer science , programming language
We have cloned a novel inward rectifier K + channel (hIRK2) from a human frontal cortex cDNA library. The amino acid sequence of hIRK2 shares 60% and 40% identity with the mouse IRK1 and the rat ROMK1 channels, respectively. Xenopus oocytes injected with hIRK2 cRNA showed an inwardly rectifying K + current that had a prominent ‘N‐shape’ I‐V curve and was blocked by extracellular Ba 2+ . The hIRK2 channel has two unique features: (a) an 18 amino acid insertion between the first transmembrane region and the pore, and (b) restricted mRNA distribution found only in human brain and heart.