Premium
Endothelin‐1 increases the levels of mRNA and protein of muscarinic acetylcholine receptors and c‐ fos mRNA in cerebellar granule cells
Author(s) -
Fukamauchi Fumihiko,
Chuang De-Maw
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00611-3
Subject(s) - muscarinic acetylcholine receptor , messenger rna , receptor , acetylcholine , medicine , endocrinology , chemistry , acetylcholine receptor , endothelin 1 , biology , microbiology and biotechnology , biochemistry , gene
Endothelin‐1 (ET‐1) induced a time‐ and dose‐dependent increase in the levels of mRNA of m 2 ‐ and m 3 ‐muscarinic acetylcholine receptors (mAChRs) in cultured cerebellar granule cells. The levels of immunoprecipitable m 3 ‐mAChR protein and total mAChR binding sites were also increased by ET‐1 treatment. The up‐regulation of m 2 ‐ and m 3 ‐mAChR mRNA was blocked by phorbol ester pretreatment to inhibit ET‐1‐stimulated phosphoinositide hydrolysis and was preceded by an increase in c‐ fos mRNA levels. Treatments that prevented ET‐1‐induced c‐ fos mRNA increase also abolished the subsequent m 2 ‐and m 3 ‐mAChR mRNA up‐regulation, suggesting that c‐Fos protein is involved in the ET‐1‐induced mAChR expression.