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Heterologous expression of cDNAs encoding barley ( Hordeum vulgare ) (1 → 3)‐β‐glucanase isoenzyme GV
Author(s) -
Xu Peilin,
Harvey Andrew J.,
Fincher Geoffrey B.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00610-5
Subject(s) - hordeum vulgare , glucanase , isozyme , heterologous expression , biology , heterologous , genetics , microbiology and biotechnology , chemistry , gene , biochemistry , botany , enzyme , poaceae , recombinant dna
Two cDNAs have been isolated from libraries generated from poly(A) + RNA of young barley roots and leaves, using a cDNA encoding barley (1 → 3)‐β‐glucanase isoenzyme GII as a probe. Nucleotide sequence analyses and ribonuclease protection assays show that the two cDNAs differ only in the length of their 3′‐untranslated regions; the corresponding mRNAs are likely to originate from a single gene by tissue‐specific processing at separate polyadenylation sites. When the coding region of the cDNA is expressed in E. coli , the resultant protein catalyses the hydrolysis of (1 → 3)‐β‐glucan with an action pattern characteristic of a (1 → 3)‐β‐glucan endohydrolase (EC 3.2.1.39). The enzyme has been designated isoenzyme GV of the barley (1 → 3)‐β‐glucanase family.