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Expression of functional Raphanus sativus antifungal protein in yeast
Author(s) -
Vilas Alves Ana L.,
De Samblanx Genoveva W.,
Terras Franky R.G.,
Cammue Bruno P.A.,
Broekaert Willem F.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00597-4
Subject(s) - raphanus , antifungal , yeast , biochemistry , chemistry , microbiology and biotechnology , biology , botany
Rs‐AFP2 is a 51 amino acid cysteine‐rich peptide isolated from radish ( Raphanus sativus ) seeds that exhibits potent inhibitory activity against filamentous fungi. A cDNA clone encoding the Rs‐AFP2 preprotein was modified by recombinant DNA methods to allow expression in the yeast Saccharomyces cerevisiae . This peptide was expressed in yeast as a fusion protein carrying at its N‐terminus the prepro‐sequences derived from the precursor of the yeast pheromone mating factor α1. These sequences allow secretion of the biologically active peptide in a correctly processed form. Deletion of the mating factor α1 pro‐peptide drastically reduced the expression level of the peptide.