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Expression of the barley psbA gene in Escherichia coli yields a functional in vitro photosystem II protein D1
Author(s) -
Efimov Vladimir A.,
Fradkov Arkady F.,
Raskind Alexander B.,
Khristin Michael S.,
Klimov Vyacheslav V.,
Chakhmakhcheva Oksana G.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00586-9
Subject(s) - escherichia coli , chloroplast , histidine , biology , photosystem ii , gene , thylakoid , biochemistry , microbiology and biotechnology , recombinant dna , enzyme , photosynthesis
The barley chloroplast psbA gene encoding D1 protein, one of the main photosystem II components, has been over‐expressed in E. coli cells. The existance of two in vivo expression products, a protein with M r about 33.5 kDa, corresponding to the full‐length precursor of the 32 kDa D1 mature form, and a truncated 29 kDa polypeptide was revealed. A modified D1 protein containing six histidine residues at the carboxy‐terminus was also obtained. After isolation and renaturation, the ability of the recombinant D1 protein to bind atrazine and pigments from barley thylakoids was demonstrated.