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8‐Hydroxydeoxyguanosine
Author(s) -
Lunec Joseph,
Herbert Karl,
Blount Susan,
Griffiths Helen R.,
Emery Paul
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00583-4
Subject(s) - urine , immune system , urinary system , dna damage , chemistry , dna , oxidative damage , immunology , lupus erythematosus , antigen , systemic lupus erythematosus , medicine , endocrinology , biochemistry , antibody , oxidative stress , disease
8‐Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 8OHDG by HPLC. Further analysis by GC‐MS confirmed that levels of 8OHDG in SLE urine were 10 3 ‐fold lower than in an age‐ and sex‐matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H 2 O 2 confirmed the impaired ability of SLE lymphocytes to repair 8OHDG. We subsequently observed in SLE patients that 8OHDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.