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Loss of phosphatidylserine synthesis results in aberrant solute sequestration and vacuolar morphology in Saccharomyces cerevisiae
Author(s) -
Hamamatsu Shioka,
Shibuya Isao,
Takagi Masamichi,
Ohta Akinori
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00576-1
Subject(s) - phosphatidylserine , saccharomyces cerevisiae , morphology (biology) , chemistry , microbiology and biotechnology , biophysics , biochemistry , biology , zoology , yeast , membrane , phospholipid
Null cho1 mutants of Saccharomyces cerevisiae are incapable of phosphatidyl‐serine synthesis. They were more susceptible than wild‐type strains to 100 mM CaCl 2 , 3 mM ZnCl 2 or 1 mM MnCl 2 , but not to MgCl 2 nor KCl. They were also susceptible to high concentrations of basic amino acids, l ‐lysine and l ‐arginine, and to an l ‐lysine analog, S ‐2‐aminoethyl‐ l ‐cysteine. Their vacuolar pools of amino acids, especially those of basic ones, were decreased. Pigmentation of chol ade2 double mutants was obscured and vacuoles of cho1 mutants were considerably fragmented. These indicate that phosphatidylserine plays vital roles in normal vacuolar function and morphogenesis.