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Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G αq and not G α11
Author(s) -
Shapira Hagit,
Way James,
Lipinsky Dafna,
Oron Yoram,
Battey James F.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00570-2
Subject(s) - xenopus , bombesin , receptor , microbiology and biotechnology , biology , gq alpha subunit , g protein , oocyte , g alpha subunit , endocrinology , medicine , protein subunit , chemistry , neuropeptide , gene , biochemistry , embryo
G‐proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus G αq and G α11 and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. G αq antisense inhibited responses mediated by neuromedin B receptor (NMB‐R) by 74%, though not by gastrin‐releasing peptide receptor (GRP‐R). G α11 antisense had little effect on either GRP‐R‐ or NMB‐R‐mediated responses. This suggests that NMB‐R couples to G αq , and that GRP‐R and NMB‐R show distinct G‐protein coupling preferences in the Xenopus oocyte.