Premium
Ca 2+ ‐dependent cosecretion of adrenomedullin and catecholamines mediated by nicotinic receptors in bovine cultured adrenal medullary cells
Author(s) -
Katoha Fumi,
Niina Hiromi,
Kitamura Kazuo,
Ichiki Yoshinari,
Yamamoto Yoshitaka,
Kangawa Kenji,
Eto Tanenao,
Wada Akihiko
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00566-4
Subject(s) - adrenomedullin , medicine , endocrinology , catecholamine , nicotinic agonist , secretion , receptor , chemistry , adrenal medulla , muscarinic acetylcholine receptor , chromaffin cell , biology
Bovine cultured adrenal medullary cells (4 × 10 6 ) contained 4266.5 ± 370.0 fmol of immunoreactive adrenomedullin and 373.4 ± 32.6 mnol of catecholamines. Nicotinic (but not muscarinic) receptors mediated the Ca 2+ ‐dependent co‐secretion of adrenomedullin and catecholamines, with the molar ratio of adrenomedullin/catecholamines secreted into the medium being equal to the ratio stored in the cells. The concentration‐response curve of carbachol for adrenomedullin secretion (EC 50 42 μM) was similar to that for catecholamine secretion (EC 50 63 μM). Reverse phase HPLC analysis showed that immunoreactive adrenomedullins in the cells and secreted into the medium were both eluted exclusively at the position almost identical to synthetic human adrenomedullin[1–52]NH 2 .