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Distinguishing between duplex and hairpin forms of RNA by 15 N– 1 H heteronuclear NMR
Author(s) -
Aboul-ela Fareed,
Nikonowicz Edward P.,
Pardi Arthur
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00564-8
Subject(s) - heteronuclear molecule , duplex (building) , rna , chemistry , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , physics , crystallography , dna , biochemistry , gene
A general method is described for distinguishing RNA hairpins from RNA duplexes by application of two‐dimensional filtered nuclear Overhauser enhancement spectra on a 1:1 mixture of unlabeled and 99% 15 N‐labeled molecules. The method is applied to the RNA dodecamer rGGCGCUUGCGUC which can form an intramolecular hairpin under low salt conditions and a duplex in high salt. This procedure allows unambiguous identification of RNA hairpins or duplexes under the same conditions that are used in the NMR solution structure determination.