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Myelin membrane biogenesis by oligodendrocytes Developmental regulation of low molecular weight GTP‐binding proteins
Author(s) -
Huber Lukas A.,
Madison Dana L.,
Simons Kai,
Pfeiffer S.E.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00562-1
Subject(s) - myelin , oligodendrocyte , microbiology and biotechnology , biology , biogenesis , gtp' , gtp binding protein regulators , membrane protein , proteolipid protein 1 , biochemistry , g protein , chemistry , myelin basic protein , membrane , signal transduction , neuroscience , central nervous system , enzyme , gene
Oligodendrocytes synthesize dramatic amounts of myelin membrane. We hypothesized that this requires unique aspects of vesicular trafficking. Specific stages of the oligodendrocyte lineage were assayed for low molecular weight GTP‐binding proteins implicated in the regulation of vesicular transport pathway (two dimensional gel electrophoresis, [α‐ 32 P]GTP overlay). Consistent with the hypothesis, as oligodendrocytes differentiate from early progenitors to mature myelin‐producing cells, ≥≥ 12 small GTP‐binding proteins become up‐regulated. Myelin membrane also has a complex pattern of GTP‐binding proteins. Several of these proteins may be specific to oligodendrocytes, suggesting that oligodendrocytes may utilize cell‐type specific GTP‐binding proteins for biogenesis and maintenance of the myelin membrane.