Premium
Free radical activation of acetaldehyde and its role in protein alkylation
Author(s) -
Albanoa Emanuele,
Clot Paolo,
Comoglio Adriana,
Dianzani Mario U.,
Tomasi Aldo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00549-4
Subject(s) - chemistry , radical , acetaldehyde , superoxide dismutase , catalase , spin trapping , photochemistry , adduct , xanthine oxidase , medicinal chemistry , biochemistry , organic chemistry , ethanol , enzyme
The formation of carbon centered free radicals, identified as methylcarbonyl species, was observed using ESR spectroscopy and the spin trapping agent 4‐pyridyl‐1‐oxide‐ N ‐ t ‐butyl nitrone (4‐POBN) during the oxidation of acetaldehyde by xanthine oxidase. The reaction was dependent upon the presence of OH • radicals and was inhibited by the addition of superoxide dismutase, catalase or OH • radical scavengers. The generation of methylcarbonyl radicals was associated with a doubling of stable acetaldehyde adducts with serum albumin, and 4‐POBN or superoxide dismutase and catalase, completely blocked this effect. Thus, methylcarbonyl radicals contributed to acetaldehyde‐mediated protein alkylation which is involved in causing toxic as well as immunological reactions ascribed to acetaldehyde.