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Yeast serine isoacceptor tRNAs: Variations of their content as a function of growth conditions and primary structure of the minor tRNA Ser GCU
Author(s) -
Heyman Thérèse,
Agoutin Bernadette,
Fix Christine,
Dirheimer Guy,
Keith Gérard
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00524-9
Subject(s) - transfer rna , yeast , chemistry , serine , biochemistry , rna , enzyme , gene
The primary structure of Saccharomyces cerevisiae tRNA Ser GCU is presented (EMBL database accession No. X74268 S. cerevisiae tRNA‐Ser). In addition, quantitation of the relative amounts of serine isoaccepting tRNAs in yeast grown on different media showed that the minor tRNA Ser GCU decreased while the major tRNA Ser AGA increased as the growth rate and the cellular protein content increased. The minor species, tRNA Ser CGA and tRNA Ser UGA, were not separated by our gel system, however, taken together they appeared to vary in the same way as TRNA Ser GCU. These data suggest a growth rate dependence of yeast tRNAs similar to that previously described for E. coli tRNAs.