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Human chromosomal centromere (AATGG) n sequence forms stable structures with unusual base pairs
Author(s) -
Jaishree T.N.,
Wang Andrew H.-J.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00516-8
Subject(s) - centromere , base pair , duplex (building) , crystallography , dna , nuclear magnetic resonance spectroscopy , sequence (biology) , chemistry , stereochemistry , biology , genetics , chromosome , gene
Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG) n ·(CCAAT) n repeat have been studied by two‐dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG) n sequence has an equilibrium between the duplex form and a fold‐back form. Structural refinement of d(CAATGG) and its related sequences by an NOE‐constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type‐I G‐A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.