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Adenovirus‐mediated gene transfer to murine retinal cells in vitro and in vivo
Author(s) -
Jomary C.,
Piper T.A.,
Dickson G.,
Couture L.A.,
Smith A.E.,
Neal M.J.,
Jones S.E.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00512-5
Subject(s) - in vivo , in vitro , biology , viral vector , microbiology and biotechnology , retinal , retinal pigment epithelium , genetic enhancement , adenoviridae , cell culture , recombinant dna , gene , genetics , biochemistry
Adenovirus‐mediated gene transfer to retinal cells was evaluated using the replication‐defective recombinant adenovirus vector Ad2/CMV lacZ ‐1 (coding for β‐galactosidase) both in an in vitro murine culture model and in vivo in adult mice. In vitro, no difference in infectability of neuronal and glial cells was observed, and 50% of neurons expressed the exogenous gene at low viral concentration (10 pfu/cell). In vivo, intraocular injection of 3 × 10 6 pfu Ad2/CMV lacZ ‐1 resulted in expression of the transferred β‐galactosidase gene in retinal pigment epithelium and ganglion cells. These results demonstrate that Ad2/CMV lacZ ‐1 is an effective vector for gene transfer into retinal cells.