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Parallel changes in Glut 4 and Rab4 movements in two insulin‐resistant states
Author(s) -
Ricort Jean-Marc,
Tanti Jean-François,
Cormont Mireille,
Van Obberghen Emmanuel,
Le Marchand-Brustel Yannick
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00510-9
Subject(s) - insulin , intracellular , medicine , insulin resistance , endocrinology , glucose transporter , chromosomal translocation , stimulation , biology , compartment (ship) , diabetes mellitus , insulin oscillation , chemistry , microbiology and biotechnology , biochemistry , oceanography , geology , gene
Insulin‐induced Glut 4 and Rab4 movements were studied in two insulin‐resistant states. In adipocytes from streptozotocin diabetic rats, the amount of Glut 4 was decreased by 60%. The remaining Glut 4 molecules were translocated in response to insulin, and in parallel, Rab4 left the intracellular compartment. In contrast, in 3T3‐L1 adipocytes rendered insulin‐resistant by a prolonged insulin treatment, both Rab4 and Glut 4 remained in the intracellular compartment following an acute insulin stimulation. Those results illustrate a similar behavior of Glut 4 and Rab4 in two situations where insulin resistance results from different mechanisms, and add further support for a role of Rab4 in Glut 4 transiocation.