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Vitamin D status modulates rat colonic M 3 muscarinic receptor characteristics and coupling to guanylate cyclase
Author(s) -
Khare Sharad,
Tien Xiao-Ying,
Wilson David,
Wali Ramesh K.,
Bissonnette Bruce M.,
Sitrin Michael D.,
Brasitus Thomas A.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00498-6
Subject(s) - quinuclidinyl benzilate , carbachol , endocrinology , chemistry , medicine , muscarinic acetylcholine receptor , dissociation constant , stimulation , receptor , vitamin , guanylate cyclase 2c , membrane , gucy1b3 , guanylate cyclase , biochemistry , biology
The present studies were conducted to determine whether [ 3 H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol‐mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC 50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D‐deficient rats compared to their D‐sufficient counterparts. Concomitantly, the density of receptors ( B max ) were significantly lower, and dissociation constants ( K d ) were significantly higher in D‐deficient colonic membranes. In vitamin D‐repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.