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Identification of the GABA A receptor subtype mRNA in human pancreatic tissue
Author(s) -
Yang Wu,
Antonio A. Reyes,
Nancy C. Lan
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00485-4
Subject(s) - receptor , pancreas , biology , messenger rna , glutamate receptor , reverse transcriptase , reverse transcription polymerase chain reaction , endocrinology , medicine , enzyme , microbiology and biotechnology , biochemistry , polymerase chain reaction , gene
Evidence suggests a physiological role of the GABA A receptor in the pancreas. Clinically, an autoimmune reaction involving the GABA biosynthesizing enzyme, glutamic acid decar☐ylase has been implicated in the development of insulin‐dependent diabetes mellitus. To determine the subtypes of GABA A receptor expressed in human pancreas, we analyzed, with the use of the reverse‐transcription/polymerase chain reaction technique human pancreatic tissue for the presence of GABA A receptor subunits α1–6, β1–3, and γ1–2 transcripts. Unlike brain tissue, pancreatic tissue only expresses the α2, β3 and γ1 subunits. Our results provide evidence of a specific GABA A receptor subtype expressed in human pancreatic tissue.