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Activation of human neutrophils by C3a and C5A Comparison of the effects on shape changes, chemotaxis, secretion, and respiratory burst
Author(s) -
Markus U. Ehrengruber,
Thomas Geiser,
David A. Deranleau
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00463-3
Subject(s) - chemotaxis , anaphylatoxin , desensitization (medicine) , respiratory burst , receptor , stimulation , respiratory system , secretion , complement component 5 , immunology , homologous desensitization , microbiology and biotechnology , chemistry , biology , biophysics , endocrinology , complement system , biochemistry , anatomy , immune system
The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50‐ to 100‐times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with C5a, but no neutrophil chemotaxis was observed with C3a. Repeated stimulation led to homologous desensitization of shape changes and respiratory burst but no cross‐desensitization, indicating that the two anaphylatoxins act through separate receptors. The lack of chemotactic activity suggests that C3a is not involved in neutrophil recruitment into infected or inflamed tissues.