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Cyclic GMP in the perfused rat heart Effect of ischaemia, anoxia and nitric oxide synthase inhibitor
Author(s) -
Depré Christophe,
Hue Louis
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00459-5
Subject(s) - glycogenolysis , nitric oxide , nitric oxide synthase , glycogen phosphorylase , medicine , chemistry , ischemia , endocrinology , perfusion , glycogen
Working rat hearts perfused with 5.5 mM glucose were submitted to a 10‐min period of no‐flow ischaemia or anoxia. Both conditions stimulated glycogenolysis, activated phosphorylase and increased cyclic GMP content, although the time course of these changes differed in anoxia and ischaemia. Changes in cyclic GMP content were not correlated with glycogenolysis or phosphorylase activation. Perfusion with 1 μM l ‐nitroarginine methylester, an inhibitor of nitric oxide synthase, decreased cGMP concentration under normoxic conditions and abolished the ischaemia‐induced increase in cGMP. The inhibitor decreased the coronary flow without affecting the overall working performance of the hearts under normoxic conditions.