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Sequence analysis of the promoter region of the rat somatostatin receptor subtype 1 gene
Author(s) -
Hauser Frank,
Meyerhof Wolfgang,
Wulfsen Iris,
Schönrock Christiane,
Richter Dietmar
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00444-7
Subject(s) - somatostatin receptor 1 , somatostatin receptor , somatostatin receptor 3 , somatostatin receptor 2 , microbiology and biotechnology , somatostatin , biology , gene , messenger rna , gene expression , intron , northern blot , genetics , endocrinology
Somatostatin receptor (SSTR) subtype genes are differentially expressed in brain and various peripheral tissues. RNA blotting and semiquantitative PCR analyses have revealed low levels of SSTR1 mRNA in the gastrointestinal tract and relatively high levels in GH 3 anterior pituitary cells. As a first step in the investigation of the regulation of SSTR1 gene expression, we isolated a genomic fragment that contains the promoter region and determined the transcriptional initiation site. The SSTR1 gene lacks introns and TATA and CAAT motifs, but possesses several consensus recognition sequences for the transcription factors GCF and AP‐2. The presence, also, of two Pit‐1 binding sites could explain the high SSTR1 mRNA levels in GH 3 cells.