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Phosphoinositides in membranes that build up the triads of rabbit skeletal muscle
Author(s) -
Milting Hendrik,
Heilmeyer Ludwig M.G.,
Thieleczek Rolf
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00440-4
Subject(s) - sarcolemma , skeletal muscle , endoplasmic reticulum , membrane , chemistry , phospholipid , rabbit (cipher) , biophysics , biochemistry , microbiology and biotechnology , anatomy , biology , statistics , mathematics
The total membrane concentrations of PtdIns, PtdIns4P, and PtdIns(4,5)P 2 contribute to the functional capacity of the Ins(1,4,5)P 3 signalling system which is operating in skeletal muscle but the function of which is still unknown. Total amounts of these phosphoinositides have been determined in purified membranes of transverse tubules (TT) and terminal cisternae (TC) of the sarcoplasmic reticulum (SR) of rabbit skeletal muscle. PtdIns and PtdIns4P have been detected in both membrane systems whereas PtdIns(4,5)P 2 (290 μmol/mol phospholipid) is confined only to TT. A much greater pool of PtdIns(4,5)P 2 seems, however, to be located in the sarcolemma away from the triadic junction.