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Vacuolar ATPase inactivation blocks recycling to the trans ‐Golgi network from the plasma membrane
Author(s) -
Reaves Barbara,
Banting George
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00437-4
Subject(s) - endosome , endocytic cycle , microbiology and biotechnology , organelle , golgi apparatus , bafilomycin , chemistry , ingression , membrane , cell , biology , biochemistry , endocytosis , cell division , cytokinesis , apoptosis , autophagy
TGN38/41 is an integral membrane protein which recycles between the trans ‐Golgi network (TGN) and the cell surface but is predominantly located in the TGN of rat (NRK) cells at steady state. As part of our studies on the mechanism and route of recycling between the TGN and the cell surface we have used chloroquine or Bafilomycin A 1 to modulate the lumenal pH of endocytic organelles. The data we present demonstrate that inactivation of the proton pump which maintains the acidic environment within the lumen of endocytic organelles leads to an accumulation of TGN38/41 in early endosomes. These data confirm the observation that TGN38/41 recycles between the plasma membrane and the TGN and identifies a specific block in that recycling pathway.