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Synthetic peptides in the determination of hepatitis A virus T‐cell epitopes
Author(s) -
Ivanov Vadim S.,
Kulik Liudmila N.,
Gabrielian Andrei E.,
Tchikin Leonid D.,
Kozhich Alexander T.,
Ivanov Vadim T.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00427-7
Subject(s) - epitope , capsid , virology , biology , microbiology and biotechnology , t cell , antigen , peptide , virus , chemistry , biochemistry , immunology , immune system
Computer search for probable T‐epitopes of hepatitis A virus capsid proteins was performed using an integrated set of programs. Eight segments of the VP1, VP2, VP3 and VP4 proteins were chosen and synthesised. Five peptides previously examined as probable B‐epitopes were used as well. All the peptides were tested for their ability to stimulate proliferation of lymph node T‐cells primed with synthetic peptides. Almost all predicted T‐epitopes affected the T‐cell proliferation. None of the peptides had mitogenic activity. We demonstrated that regions 17–33 and 276–298 of VP1 are possible immunodominant promiscuous sites activating lymphocytes of all mouse haplotypes.