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Expression and functional characterisation of a synthetic version of the human D 4 dopamine receptor in a stable human cell line
Author(s) -
McHale Mark,
Coldwell Martin C.,
Herrity Nicole,
Boyfield Izzy,
Winn Fiona M.,
Ball Stuart,
Cook Ted,
Robinson Jeff H.,
Gloger Israel S.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00423-4
Subject(s) - receptor , hek 293 cells , gene , transfection , dopamine receptor , microbiology and biotechnology , adenylate kinase , cell culture , g protein coupled receptor , biology , genetics
A synthetic version of the human D 4 (hD4) dopamine receptor was prepared. The G/C content of the natural gene was reduced by 14% without altering the amino acid composition of the corresponding protein sequence. HEK293 cells were transfected with the synthetic hD4 gene and stable clones resistant to G418 selected. The hD4 receptor expressed from the synthetic gene had identical pharmacological characteristics to the native hD4 receptor [(1991) Nature 350, 610–619; (1992) Nature 358, 149–152]. Functional studies with cells expressing the synthetic hD4 gene indicated negative coupling of this receptor to adenylate cyclase.