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Protein kinase C inhibitors induce apoptosis in human malignant glioma cell lines
Author(s) -
Couldwell William T.,
Hinton David R.,
He Shikun,
Chen Thomas C.,
Sebat Ibrahim,
Weiss Martin H.,
Law Ronald E.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00415-3
Subject(s) - apoptosis , glioma , cancer research , kinase , chemistry , cell culture , protein kinase a , microbiology and biotechnology , biology , biochemistry , genetics
Previous work has demonstrated the importance of the protein kinase C (PKC) system in regulating glioma growth, and has led to clinical trials utilizing PKC inhibitors as adjuncts in the therapy of patients harboring malignant gliomas. This study was performed to explore the possibility that inhibition of PKC in gliomas was triggering an apoptosis signal. Glioma cell lines were treated with PKC inhibitors staurosporine (10 nM), and tamoxifen (10 μM). DNA from cells treated with each of these drugs exhibited a ‘ladder’ pattern of oligonucleosome‐sized fragments characteristic of apoptosis, thus suggesting that in glioma cells, these drugs may be cytocidal in action.