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A mutation blocking the formation of membrane or periplasmic endogenous and exogenous c ‐type cytochromes in Escherichia coli permits the cytoplasmic formation of Hydrogenobacter thermophilus holo cytochrome c 552
Author(s) -
Sambongi Yoshihiro,
Crooke Helen,
Cole Jeff A.,
Ferguson Stuart J.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00399-8
Subject(s) - periplasmic space , paracoccus denitrificans , escherichia coli , cytochrome , biochemistry , mutant , chemistry , biology , inner membrane , membrane , enzyme , gene
A mutant of Escherichia coli , JCB606, shown to be pleiotropically deficient in the formation of endogenous membrane and periplasmic c ‐type cytochromes, synthesised the apo form of the erogenous cytochrome c 550 from Paracoccus denitrificans , but not the holo form. In contrast, a cytoplasmically located holo form of Hydrogenobacter thermophilus cytochrome c 552 was found in E. coli JCB606. These findings support the proposition that the formation of the cytoplasmic H. thermophilus cytochrome c 552 in E. coli does not involve the physiological pathway of c ‐type cytochrome biosynthesis in E. coli and that the haem insertion may be uncatalysed.