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Characterization of cytosolic malic enzyme in human tumor cells
Author(s) -
Loeber Gerhard,
Dworkin Mark B.,
Infante Anthony,
Ahorn Horst
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00386-6
Subject(s) - malic enzyme , enzyme , biochemistry , gene isoform , cytosol , recombinant dna , chemistry , malate dehydrogenase , biology , trypsin , microbiology and biotechnology , dehydrogenase , gene
Cytosolic NADP + ‐dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli . Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human ‘normal cell’ ME and NADP + ‐ME from tumor cells were identical. Thus, NADP + ‐ME found in tumor cells does not constitute a tumor‐specific isoform as suggested by other studies but is identical to the ‘housekeeping protein’ predominantly expressed in human liver and white adipose tissue.