Premium
Site‐specific incorporation of photofunctional nonnatural amino acids into a polypeptide through in vitro protein biosynthesis
Author(s) -
Hohsaka Takahiro,
Sato Ken,
Sisido Masahiko,
Takai Kazuyuki,
Yokoyama Shigeyuki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00381-5
Subject(s) - amino acid , in vitro , biosynthesis , biochemistry , chemistry , transfer rna , protein biosynthesis , enzyme , rna , gene
Nonnatural amino acids with photofunctional groups were incorporated site‐specifically into a polypeptide by using in vitro protein synthesizing system. The nonnatural amino acids were attached to tRNA CCU through chemical misacylation method, and added to the in vitro system with a mRNA containing a single AGG codon. l ‐ p ‐Phenylazophenylalanine, l ‐2‐anthrylalanine, l ‐1‐naphthylalanine, l ‐2‐naphthylalanine and l ‐ p ‐biphenylalanine were successfully incorporated into a polypeptide, but l ‐1‐pyrenylalanine was not. The polypeptides containing the nonnatural amino acids showed photofunctionalities.